Monitoring Baseline Synaptic Transmission

In most pharmacologic experiments using physiologic recordings in hippocam-pal slice preparations, the effects of drug application on baseline synaptic transmission can be evaluated by simply monitoring EPSPs before and after drug application, using a constant stimulus intensity. A more elaborate alternative is to produce input-output curves for EPSP initial slope (or magnitude) versus stimulus intensity for the presynaptic stimulus (see Figure 5).

These types of within-slice experiments are very straightforward; however, this type of within-preparation design is not possible in some experimental comparisons. For example, if one is comparing a wild-type with a knockout animal, there of necessity must be a comparison across preparations. How does one evaluate if there is a difference in basal synaptic transmission in this situation? The principal confound is that while one has control over the

Brain Battalion

Brain Battalion

Get All The Support And Guidance You Need To Be A Success At Beefing Up Your Brain. This Book Is One Of The Most Valuable Resources In The World When It Comes To Understanding Your Brain to Come Up With A Battle Plan For Intelligence.

Get My Free Ebook

Post a comment